Mapping Quality Thresholds

The plot below shows the percentages of correctly and wrongly mapped reads for all mapping quality thresholds for this mapper. The values at threshold 0 therefore correspond to the unfiltered results.

Basic Statistics
Correctly Mapped21964091.691%
Wrongly Mapped198888.302%
Not Mapped150.006%
Total239543100.0%
Read Failure Statistics
Not mapped150.006%
Missing in mapper output00.0%
Mapped to wrong chromosome105644.41%
Mapped to wrong position93243.892%
Mapped to wrong strand00.0%
Advanced Statistics
Missing in comparison alignment0
Secondary Alignments0
F-Measure0.956700
Precision0.917000
Recall0.999900
Timing
Raw Mapping Time12.288s
Effective Mapping Time12.127s
Effective Init Time0.161s
Effective Time MeasureWall clock
Mapping Time (Wall)12.288s
Mapping Time (CPU)43.263s
Mapping Time (CPU User)42.705s
Mapping Time (CPU System)0.559s
Init Time (Wall)0.161s
Init Time (CPU)0.167s
Init Time (CPU User)0.049s
Init Time (CPU System)0.118s
Additional Information
Mapper Memory Usage239 MB
Total Test Runtime (Wall)226.496s
Mapper Command Line: 
/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads.fastq -S out_bowtie2.sam
Errors and Warnings
No problems were encountered.
Subprocess Log

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2-build /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2-build /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt
return1
status1
stderr
None
stdout
Settings:
Output files: "/project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt.*.bt2"
Line rate: 6 (line is 64 bytes)
Lines per side: 1 (side is 64 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
/project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta
Building a SMALL index
Reading reference sizes
Time reading reference sizes: 00:00:01
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:01
bmax according to bmaxDivN setting: 35643254
Using parameters --bmax 26732441 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 26732441 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:03
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:00
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:01
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:05
Splitting and merging
Splitting and merging time: 00:00:00
Split 1, merged 7; iterating...
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:05
Splitting and merging
Splitting and merging time: 00:00:00
Split 1, merged 0; iterating...
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:04
Splitting and merging
Splitting and merging time: 00:00:00
Split 1, merged 1; iterating...
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:05
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 1.78216e+07 (target: 26732440)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 10291629
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Sorting block time: 00:00:04
Returning block of 10291630
Getting block 2 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 17884072
(Using difference cover)
Sorting block time: 00:00:08
Returning block of 17884073
Getting block 3 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 11845640
(Using difference cover)
Sorting block time: 00:00:05
Returning block of 11845641
Getting block 4 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 23535943
(Using difference cover)
Sorting block time: 00:00:09
Returning block of 23535944
Getting block 5 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 25546130
(Using difference cover)
Sorting block time: 00:00:10
Returning block of 25546131
Getting block 6 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 24195086
(Using difference cover)
Sorting block time: 00:00:10
Returning block of 24195087
Getting block 7 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 15510888
(Using difference cover)
Sorting block time: 00:00:06
Returning block of 15510889
Getting block 8 of 8
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 13763622
(Using difference cover)
Sorting block time: 00:00:06
Returning block of 13763623
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 41351059
fchr[G]: 71311743
fchr[T]: 101240555
fchr[$]: 142573017
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 51892598 bytes to primary EBWT file: /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt.1.bt2
Wrote 35643260 bytes to secondary EBWT file: /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 142573017
bwtLen: 142573018
sz: 35643255
bwtSz: 35643255
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 8910814
offsSz: 35643256
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 742568
numLines: 742568
ebwtTotLen: 47524352
ebwtTotSz: 47524352
color: 0
reverse: 0
Total time for call to driver() for forward index: 00:01:46
Reading reference sizes
Time reading reference sizes: 00:00:01
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:01
Time to reverse reference sequence: 00:00:00
bmax according to bmaxDivN setting: 35643254
Using parameters --bmax 26732441 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 26732441 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:03
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:00
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:01
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:05
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 2.03676e+07 (target: 26732440)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 18299241
(Using difference cover)
Sorting block time: 00:00:08
Returning block of 18299242
Getting block 2 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 26126377
(Using difference cover)
Sorting block time: 00:00:11
Returning block of 26126378
Getting block 3 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 21131594
(Using difference cover)
Sorting block time: 00:00:08
Returning block of 21131595
Getting block 4 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 22948118
(Using difference cover)
Sorting block time: 00:00:09
Returning block of 22948119
Getting block 5 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 21030658
(Using difference cover)
Sorting block time: 00:00:08
Returning block of 21030659
Getting block 6 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 21802436
(Using difference cover)
Sorting block time: 00:00:09
Returning block of 21802437
Getting block 7 of 7
Reserving size (26732441) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:02
Sorting block of length 11234587
(Using difference cover)
Sorting block time: 00:00:04
Returning block of 11234588
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 41351059
fchr[G]: 71311743
fchr[T]: 101240555
fchr[$]: 142573017
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 51892598 bytes to primary EBWT file: /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt.rev.1.bt2
Wrote 35643260 bytes to secondary EBWT file: /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt.rev.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 142573017
bwtLen: 142573018
sz: 35643255
bwtSz: 35643255
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 8910814
offsSz: 35643256
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 742568
numLines: 742568
ebwtTotLen: 47524352
ebwtTotSz: 47524352
color: 0
reverse: 1
Total time for backward call to driver() for mirror index: 00:01:31
systime0
time197.714474916
usrtime0
working_directory/project2/NextGenMapTest/Teaser/tests_generated/D1_n

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads_base.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads_base.fastq -S out_bowtie2.sam
memory220192000
return0
status1
stderr
None
stdout
1 reads; of these:
1 (100.00%) were unpaired; of these:
1 (100.00%) aligned 0 times
0 (0.00%) aligned exactly 1 time
0 (0.00%) aligned >1 times
0.00% overall alignment rate
systime0.164974
time1.26761507988
usrtime0.054991
working_directory/project2/NextGenMapTest/Teaser/tests_generated/D1_n

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads_base.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads_base.fastq -S out_bowtie2.sam
memory219840000
return0
status1
stderr
None
stdout
1 reads; of these:
1 (100.00%) were unpaired; of these:
1 (100.00%) aligned 0 times
0 (0.00%) aligned exactly 1 time
0 (0.00%) aligned >1 times
0.00% overall alignment rate
systime0.117982
time0.16149020195
usrtime0.048992
working_directory/project2/NextGenMapTest/Teaser/tests_generated/D1_n

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/d_melanogaster.fasta_bt -U reads.fastq -S out_bowtie2.sam
memory239336000
return0
status1
stderr
None
stdout
239543 reads; of these:
239543 (100.00%) were unpaired; of these:
15 (0.01%) aligned 0 times
197864 (82.60%) aligned exactly 1 time
41664 (17.39%) aligned >1 times
99.99% overall alignment rate
systime0.558915
time12.2880120277
usrtime42.704507
working_directory/project2/NextGenMapTest/Teaser/tests_generated/D1_n