Mapping Quality Thresholds

The plot below shows the percentages of correctly and wrongly mapped reads for all mapping quality thresholds for this mapper. The values at threshold 0 therefore correspond to the unfiltered results.

Basic Statistics
Correctly Mapped14445896.305%
Wrongly Mapped55363.691%
Not Mapped60.004%
Total150000100.0%
Read Failure Statistics
Not mapped60.004%
Missing in mapper output00.0%
Mapped to wrong chromosome00.0%
Mapped to wrong position55363.691%
Mapped to wrong strand00.0%
Advanced Statistics
Missing in comparison alignment0
Secondary Alignments0
F-Measure0.981200
Precision0.963100
Recall1.000000
Timing
Raw Mapping Time3.402s
Effective Mapping Time3.238s
Effective Init Time0.164s
Effective Time MeasureWall clock
Mapping Time (Wall)3.402s
Mapping Time (CPU)10.479s
Mapping Time (CPU User)10.191s
Mapping Time (CPU System)0.288s
Init Time (Wall)0.164s
Init Time (CPU)0.144s
Init Time (CPU User)0.041s
Init Time (CPU System)0.103s
Additional Information
Mapper Memory Usage73 MB
Total Test Runtime (Wall)18.478s
Mapper Command Line: 
/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads.fastq -S out_bowtie2.sam
Errors and Warnings
No problems were encountered.
Subprocess Log

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2-build /project2/NextGenMapTest/Teaser/references/E_coli.fasta /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2-build /project2/NextGenMapTest/Teaser/references/E_coli.fasta /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt
return1
status1
stderr
None
stdout
Settings:
Output files: "/project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt.*.bt2"
Line rate: 6 (line is 64 bytes)
Lines per side: 1 (side is 64 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
/project2/NextGenMapTest/Teaser/references/E_coli.fasta
Building a SMALL index
Reading reference sizes
Time reading reference sizes: 00:00:00
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:00
bmax according to bmaxDivN setting: 1283017
Using parameters --bmax 962263 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 962263 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:00
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:00
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:00
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:00
Splitting and merging
Splitting and merging time: 00:00:00
Split 1, merged 6; iterating...
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:00
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 733152 (target: 962262)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 583290
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Sorting block time: 00:00:00
Returning block of 583291
Getting block 2 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 841267
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Sorting block time: 00:00:00
Returning block of 841268
Getting block 3 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 953385
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 953386
Getting block 4 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 538875
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 538876
Getting block 5 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 949978
(Using difference cover)
Sorting block time: 00:00:01
Returning block of 949979
Getting block 6 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 912888
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Sorting block time: 00:00:00
Returning block of 912889
Getting block 7 of 7
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 352379
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 352380
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 1268010
fchr[G]: 2573696
fchr[T]: 3866403
fchr[$]: 5132068
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 5905266 bytes to primary EBWT file: /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt.1.bt2
Wrote 1283024 bytes to secondary EBWT file: /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 5132068
bwtLen: 5132069
sz: 1283017
bwtSz: 1283018
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 320755
offsSz: 1283020
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 26730
numLines: 26730
ebwtTotLen: 1710720
ebwtTotSz: 1710720
color: 0
reverse: 0
Total time for call to driver() for forward index: 00:00:02
Reading reference sizes
Time reading reference sizes: 00:00:00
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:00
Time to reverse reference sequence: 00:00:00
bmax according to bmaxDivN setting: 1283017
Using parameters --bmax 962263 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 962263 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:00
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:00
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:00
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:01
Splitting and merging
Splitting and merging time: 00:00:00
Split 2, merged 5; iterating...
Binary sorting into buckets
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Binary sorting into buckets time: 00:00:00
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 641508 (target: 962262)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 245339
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 245340
Getting block 2 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 855428
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 855429
Getting block 3 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 880204
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 880205
Getting block 4 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:01
Sorting block of length 486769
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 486770
Getting block 5 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 739485
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 739486
Getting block 6 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 406763
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 406764
Getting block 7 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 904773
(Using difference cover)
Sorting block time: 00:00:00
Returning block of 904774
Getting block 8 of 8
Reserving size (962263) for bucket
Calculating Z arrays
Calculating Z arrays time: 00:00:00
Entering block accumulator loop:
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Block accumulator loop time: 00:00:00
Sorting block of length 613300
(Using difference cover)
Sorting block time: 00:00:01
Returning block of 613301
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 1268010
fchr[G]: 2573696
fchr[T]: 3866403
fchr[$]: 5132068
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 5905266 bytes to primary EBWT file: /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt.rev.1.bt2
Wrote 1283024 bytes to secondary EBWT file: /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt.rev.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 5132068
bwtLen: 5132069
sz: 1283017
bwtSz: 1283018
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 320755
offsSz: 1283020
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 26730
numLines: 26730
ebwtTotLen: 1710720
ebwtTotSz: 1710720
color: 0
reverse: 1
Total time for backward call to driver() for mirror index: 00:00:03
systime0
time5.31316900253
usrtime0
working_directory/project2/NextGenMapTest/Teaser/tests_generated/ecoli_test

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads_base.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads_base.fastq -S out_bowtie2.sam
memory62160000
return0
status1
stderr
None
stdout
1 reads; of these:
1 (100.00%) were unpaired; of these:
1 (100.00%) aligned 0 times
0 (0.00%) aligned exactly 1 time
0 (0.00%) aligned >1 times
0.00% overall alignment rate
systime0.100984
time0.541434049606
usrtime0.042993
working_directory/project2/NextGenMapTest/Teaser/tests_generated/ecoli_test

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads_base.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads_base.fastq -S out_bowtie2.sam
memory61632000
return0
status1
stderr
None
stdout
1 reads; of these:
1 (100.00%) were unpaired; of these:
1 (100.00%) aligned 0 times
0 (0.00%) aligned exactly 1 time
0 (0.00%) aligned >1 times
0.00% overall alignment rate
systime0.102984
time0.163534879684
usrtime0.040993
working_directory/project2/NextGenMapTest/Teaser/tests_generated/ecoli_test

/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads.fastq -S out_bowtie2.sam

command/project2/NextGenMapTest/Teaser/software/bowtie2/bowtie2 -p 4 -x /project2/NextGenMapTest/Teaser/references/E_coli.fasta_bt -U reads.fastq -S out_bowtie2.sam
memory73512000
return0
status1
stderr
None
stdout
150000 reads; of these:
150000 (100.00%) were unpaired; of these:
6 (0.00%) aligned 0 times
140837 (93.89%) aligned exactly 1 time
9157 (6.10%) aligned >1 times
100.00% overall alignment rate
systime0.287956
time3.40202999115
usrtime10.19145
working_directory/project2/NextGenMapTest/Teaser/tests_generated/ecoli_test