Phylogenetic information practical session for the 12th International Workshop on Virus Evolution and Molecular Epidemiology, Athens, 2006

    http://www.cibiv.at/~hschmidt/veme/


    Slides and Software

    • Slides of the PhyloInfo session.
    • The software can be downloaded from here.

    The exercise:


    1. Which of these two alignment is more useful for phylogenetic analysis (i.e., has phylogenetic information)?
      1. test46-1701.phy
      2. test64-384.phy

      Hint: To do so, we start tree-puzzle in the same directory as the dataset - enter the dataset name when prompted for. To measure the phylogenetic content of a dataset switch the type of analysis to likelihood mapping ('b') and do not group the sequences. Start the mapping analysis typing 'y'.

      Examine the result of the datasets in dataset-name.puzzle with a text editor and the likelihood mapping diagram dataset-name.eps (or with a postscript viewer like gsview.

      Add the percentages in the corners to the the amount on completely resolved quartets. Summing the in the rectangles gives you the amount of partially resolved quartets. The percentage in the center gives the number of quartets which cannot be resolved. The last number should not be high.

    2. Test for saturation with DAMBE?
      1. test46-1701.phy
      2. test64-384.phy
      Plot for the above alignments the distance-substitution diagram using DAMBE. What do we see?